The aim of the work is to study the effectiveness of complex treatment of cats with acute bacterial cholangiohepatitis. According to inclusion and exclusion criteria, the study included a cohort of cats (n = 14) with acute bacterial cholangiohepatitis. For all sick cats the pathology was confirmed by isolation of biliary culture of microorganisms by means of cholecystocentesis under ultrasonic control. Clinical, hematological, ultrasonographic, statistical methods of investigation were used in this work. Sick cats with light form of cholangiohepatitis, prescription of combinations of marbofloxacin, metronidazole, ursodeoxycholic acid, cyanocobalamin, tocopherol acetate as therapy leads to complete recovery of animals, is accompanied by a decrease in the rate of erythrocyte sedimentation, normalization of erythrocytopoiesis indices, a decrease in the activity of alanine aminotransferase and alkaline phosphatase in serum. © 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.