The aim of the work was to evaluate the effectiveness of the use of photobiomodulation therapy (PBMT) in combination with Respiro Mirtol forte in order to improve the rehabilitation period of patients after septoplasty. 92 patients underwent septoplasty under general anesthesia (55 men and 37 women, age ranged from 18 to 46 years), followed by tamponade of the nasal cavity. Patients of the 1st group did not undergo PBMT; in patients of the 2nd group, PBMT was performed 3 hours, 6 hours and 24 hours after septoplasty (infrared pulsed laser radiation, λ = 0.890 μm, P = 10 W, 2 minutes in the projection of the wings of the nose ); Patients of the 3rd group were given PBMT in the indicated regimen and Rispiro Mirtol forte 5 days before and within 10 days after septoplasty. In 48 hours, after removing the tampons, in patients of the 2nd and 3rd groups, intranasal PBMT was used with a nozzle in the red range, with λ = 0.63 μm, P = mW, for 2 minutes. Heart rate variability (HRV) was assessed: ultra-low frequencies (ULF), high frequencies (HF), low frequencies (LF) and total power of heart rate variability (HRV), pain syndrome. ULF, LF, HF, total HRV power were significantly lower in group 2, compared with group 1, but higher than in group 3. In the period from 6 to 24 hours after septoplasty, the patients of the 1st group experienced a pain syndrome of greater intensity than the patients of the 2nd and 3rd groups (p <0.001). The patients of the 3rd group had minimal pain values compared to the 2nd group. Thus, the use of PBMT in combination with Respiro Myrtol forte in the postoperative period after septoplasty against the background of nasal tamponade helps to minimize pain, reduce inflammation in the area of surgical damage, and, consequently, less pronounced changes in the autonomic nervous system in response to surgical stress.