Objective: Investigation of the effect of Zn supplementation on Zn and Se status in exercised rats. Methods: 24 male Wistar were divided into 4 groups: control, exercised, Zn-supplemented, exercised Zn-supplemented. Zn was supplemented as Zn asparaginate. Tissue Zn and Se were estimated using ICP-MS. Results: Exercise significantly decreased muscle, kidney and hair Zn; liver, heart, skeletal muscle and kidney Se levels. Exercise results in elevation of liver Zn; serum and hair Se content. Zn supplementation elevated liver, kidney, heart and hair Zn content in exercised rats.Znsupplementation increased Se stores in animals with high physical activity. Conclusions: Effect of Zn supplementation in exercised organism may be associated not only with modulation of Zn status but regulation of Se status.