ДИАГНОСТИКА НОВОГО БАКТЕРИАЛЬНОГО ПАТОГЕНА ЗЛАКОВЫХ КУЛЬТУР XANTHOMONAS ARBORICOLA МЕТОДОМ ПЦР "В РЕАЛЬНОМ ВРЕМЕНИ"

Primers and Taqman probe were designed based on the analysis of the original and Gene Bank DNA sequences of Xanthomonas arboricola for the real-time PCR detection of this pathogen. The studies were identied temperature, number and duration of cycles of amplication, concentration’s of primers and probe that makes the method more sensitive and specic for detection Xanthomonas arboricola. Sensitivity of the real time PCR for identifying Xanthomonas arboricola is not less than 30 CFU/ml.