Клиническое значение исследования фактора роста эндотелия сосудов и активатора плазминогена урокиназного типа при раке молочной железы

Иммуноферментными методами исследовано содержание VEGF в сыворотке крови и uPA в цитозоле опухолей 248 больных раком молочной железы (РМЖ) и 55 практически здоровых женщин. Не выявлено связи исходных уровней исследуемых показателей с возрастом, репродуктивной функцией и (РЭ)(РП)(HER2-neu)-рецепторным статусом опухоли. Выявлена достоверная связь уровней VEGF и uPA со стадией и степенью злокачественности РМЖ. Обнаружена корреляция между содержанием VEGF в сыворотке крови и uPA в ткани опухоли больных РМЖ T _1-2N ₀M ₀ стадий в общей группе (r = 0,67; p = 0,001), более выраженная в группе пациенток с дольковым инфильтративным раком (r = 0,92; p = 0,001), тогда как в группе больных с протоковым инфильтративным РМЖ эта зависимость была значительно слабее (r = 0,49; p = 0,01).

Clinical significance of vegf and upa evaluation in breast cancer

The tumor neoangogenesis is an attractive topic of oncology because it is known that tumor growth and development is associated with concomitant forming of mycrovessels, providing cellular breathing and feeding. Vasculoendothelial growth factor (VEGF) is one of the important neoangiogenesis activator, stimulating endotheliocytes proliferation and migration. Urokinase type plasminogen activator (uPA) is playing a key role in process of tumor invasion, contributing to the extracellular mathrix degradation and to more easy endotheliocytes migration. The aim of study - evaluation of serum VEGF levels and tumor cytosols uPA levels in early stages breast cancer patients, and the investigation of their associations with basic clinico-morphological characteristics of breast cancer. In the study 248 breast cancer patients and 55 healthy women were accepted. The blood serum content of VEGF was determined by EIA standarts kits “Quantikine human VAGF” (“R&D Systems Inc.”, USA). The uPA level in cytosol fraction of tumor was evaluated by EIA reactives, created by Dr. T.Benraad (Nymegen, Netherlands). The reliable association between VEGF and uPA levels and stage of disease and or malignancies degree was found by the multivariate analysis. Also association between serum VEGF and tumor cytosols uPA levels in T1 and T2 stages was shown in general group of patients (r=0,67; p=0,001), this correlation was more expressed in lobular infiltrative breast cancer (r=0,92; p=0,001, and significantly less expressed in ductal infiltrative breast cancer (r=0,49; p=0,01). Obtained data support the hypothesis about engagement of VEGF-depending neoangiogenesis and also about its association with urokinase type system plasminogen activation in early stages breast cancer.