A genetically encoded Mango II-based system with increased brightness and selectivity for imaging RNAs in live cells

Few genetically encoded systems based on aptamer-dye pairs meet all the requirements of bioimaging in terms of brightness, contrast, etc., thus necessitating their optimization. Here, we report a new biotin-free fluorogenic ligand that demonstrates increased selectivity for Mango II over other DNA/RNA structures in tube and brightness in complex with genetically encoded aptamer-labeled RNA in live cells compared to the known dye. © 2025 The Royal Society of Chemistry.

Авторы
Svetlova Julia I. 1 , Bychenko Oksana S. 2 , Slushko Georgy K. 2, 3 , Skvortsova Yulia V. 2 , Belyaev E.S. 4 , Kamzeeva Polina N. 2 , Khodarovich Yu M. 2, 5 , Pakhomov Alexey A. 2 , Azhikina Tatyana L. 2 , Varizhuk Anna M. 1 , Aralov Andrey V. 1, 2, 5
Journal
Organic and Biomolecular Chemistry
Издательство
Royal Society of Chemistry
Номер выпуска
36
Язык
English
Страницы
8193-8198
Статус
Published
Ссылка
Внешняя ссылка
DOI
10.1039/D5OB01004G
Том
23
Год
2025
Организации
  • 1 Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russian Federation
  • 2 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation
  • 3 Mendeleev University of Chemical Technology of Russia, Moscow, Moscow Oblast, Russian Federation
  • 4 A.N. Frumkin Institute of Physical Chemistry and Electrochemistry of the Russian Academy of Sciences, Moscow, Russian Federation
  • 5 RUDN University, Moscow, Moscow Oblast, Russian Federation
Ключевые слова
Bioimaging; Gene encoding; Optimization; RNA; Aptamers; Bio-imaging; Dye pairs; Fluorogenics; Live cell; Optimisations; RNA structures; Luminance; aptamer; fluorescent dye; xanthone derivative; chemistry; fluorescence imaging; genetics; HeLa cell line; human; Aptamers, Nucleotide; Fluorescent Dyes; HeLa Cells; Humans; Optical Imaging; Xanthones
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